{Inland freshwaters are of great importance for human health and activities, but major stressors such as nutrient pollution, deforestation, and urbanization are compromising their status. Water quality degradation and freshwater ecosystem preservation are current issues worldwide requiring frequent and efficient monitoring protocols. The increasing need for large amounts of data to comply with national and international regulations on water quality monitoring highlights traditional procedures limits. Therefore, the purpose of the present study is to investigate the potential of alternative and rapid methods for chlorophyll concentration surveys in freshwaters. The Phyto-PAM (pulse amplitude-modulated) instrument and the Case-2 Regional Coast Colour (C2RCC) satellite image processor were selected to estimate chlorophyll concentration in the surface waters of Lake Albano (Central Italy), selected as a pilot area for the project BLOOWATER (Water JPI 2018 Joint Call Closing the Water Cycle Gap). The correlation tests’ results indicate significant relations with chlorophyll data measured spectrophotometrically, confirming the suitability of both methods for chlorophyll retrieval. However, the relatively low strength of the correlation between remotely sensed and spectrophotometric data (r = 0.57, p < 2.2 × 10−16 ) was not as satisfactory as with Phyto-PAM values (r = 0.97

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