| Title | Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2026 |
| Authors | Mazzucotelli, Elisabetta, Forestan Cristian, Zastrow-Hayes Gina, Swarbreck David, Lev-Mirom Yael, Cavalet-Giorsa Emile, Bozzoli Matteo, Mastrangelo Anna Maria, Marone Daniela, Ranwez Vincent, Girodolle Johanna, Llaca Victor, Fengler Kevin, Harris Charlotte, Toegelová Helena, Navrátilová Pavla, Faccioli Primetta, Desiderio Francesca, Valladares Ana Paola, Kaithakottil Gemy, Rusholme-Pilcher Rachel L., Spannagl Manuel, Gundlach Heidrun, Mayer Klaus F. X., Blake Victoria C., Faris Justin D., Xu Steven S., Sen Taner Z., Yao Eric, Isidro y Sánchez Julio, Liu Chunyi, Farooq Muhammad A., Stefanelli Sandra, Cappucci Chiara, Oren Leah, Eilam Tamar, Giorgioni Mario, Stella Alessandra, Lazzari Barbara, Lauria Massimiliano, Ceriotti Aldo, Pirona Raul, Tavakol Elahe, Klymiuk Valentyna, Ens Jennifer, Chawla Harmeet Singh, Walkowiak Sean, Pecchioni Nicola, Bassi Filippo M., Sanchez-Garcia Miguel, Sonnante Gabriella, Curci Pasquale L., Giuliano Giovanni, Aprea Giuseppe, Pasha Asher, Gadaleta Agata, Marcotuli Ilaria, Masci Stefania, Sestili Francesco, Palombieri Samuela, Scaglione Davide, Morgante Michele, Simková Hana, Provart Nicholas J., Krattinger Simon G., Pozniak Curtis J., Chantret Nathalie, Hall Anthony, Distelfeld Assaf, Tuberosa Roberto, Maccaferri Marco, and Cattivelli Luigi |
| Journal | Plant Biotechnology Journal |
| Type of Article | Article |
| ISSN | 14677644 |
| Abstract | Advancements in plant genome sequencing and assembly have enabled the production of increasingly accurate and contiguous genome sequences. Here, we present the chromosome-level assembly of the durum wheat (Triticum turgidum L. ssp. durum, cv. Svevo) reference genome produced using accurate long-reads, optical mapping and Hi-C. The new assembly (Svevo Rel.2.0) comprises 263 hybrid scaffolds with an N50 value of 112.3 Mb, arranged into 14 contiguous pseudomolecules spanning 10.4 Gb. The Svevo Rel.2.0 genome assembly was annotated using extensive short- and long-read RNA sequencing data obtained from 60 tissue/treatment combinations. The resulting annotation comprises 68 154 high-confidence protein-coding genes, which have been integrated into a comprehensive transcriptome atlas accessible through an eFP browser. Annotation was manually curated for storage protein gene families and for Leucine-Rich Repeat-Containing Receptor genes yielding 3763 LRR-CR loci. The genome assembly's accuracy and completeness were demonstrated by the correct reconstruction of the physical map of Tg1-B (Tenacious glumes 1), a locus controlling the free threshing trait located on chromosome 2B that was not assembled in the previous genome release (Svevo Rel.1.0). A wealth of 6621 QTLs/MTAs from the literature were mapped onto Svevo Rel.2.0 to identify QTL hotspots and trait-specific candidate genes. The ancestry of the durum genome to representative wild emmer populations from North-Eastern and Southern-Levant Fertile Crescent assessed by tracing haplotype transmission patterns revealed a clear mosaic pattern. This new durum reference genome, enhanced with advanced annotation and an expression atlas linked to QTLome data, is the most comprehensive tool available for durum wheat genomics. © 2026 The Author(s). Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA. |
| Notes | Cited by: 0; All Open Access; Gold Open Access |
| URL | https://www.scopus.com/pages/publications/105044456770?origin=resultslist |
| DOI | 10.1111/pbi.70673 |
| Citation Key | Mazzucotelli2026 |
