| Title | Bacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing. |
|---|
| Publication Type | Articolo su Rivista peer-reviewed |
|---|
| Year of Publication | 2015 |
|---|
| Authors | Fantini, Elio, Gianese Giulio, Giuliano Giovanni, and Fiore Alessia |
|---|
| Journal | Methods in molecular biology (Clifton, N.J.) |
|---|
| Volume | 1231 |
|---|
| Pagination | 77-90 |
|---|
| Date Published | 2015 |
|---|
| ISSN | 1940-6029 |
|---|
| Keywords | 16S, Agar Gel, agar gel electrophoresis, amplicon, article, Bacteria, Bacteria (microorganisms), Bacterial, bacterial gene, bacterial genome, bacterial metabarcoding, bacterial RNA, bacterium, biochip, chemistry, chromosome map, Chromosome Mapping, devices, DNA, DNA barcoding, DNA Primers, DNA sequence, Electrophoresis, Gene Library, Genes, genetics, Genome, genome analysis, Genomic Library, high throughput sequencing, High-Throughput Nucleotide Sequencing, ion torrent amplicon sequencing, metagenomics, Microbial diversity, molecular genetics, Molecular Sequence Annotation, nonhuman, Polymerase Chain Reaction, primer DNA, procedures, Productivity, proton, Protons, reference database, Ribosomal, RNA, RNA 16S, RNA sequence, Sequence Analysis, statistics and numerical data, synthesis, Taxonomic, taxonomic rank |
|---|
| Abstract | Ion Torrent is a next generation sequencing technology based on the detection of hydrogen ions produced during DNA chain elongation; this technology allows analyzing and characterizing genomes, genes, and species. Here, we describe an Ion Torrent procedure applied to the metagenomic analysis of 16S rRNA gene amplicons to study the bacterial diversity in food and environmental samples.
|
|---|
| Notes | cited By 10
|
|---|
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84914126910&doi=10.1007%2f978-1-4939-1720-4_5&partnerID=40&md5=dc54c53bd87f805ea2717f3012ab7a26 |
|---|
| DOI | 10.1007/978-1-4939-1720-4_5 |
|---|
| Citation Key | 4936 |
|---|
